Pectin test

Rapid protocol

Why the pectin test matters

Juice clarification (static settling or flotation) requires a complete degradation of pectin. A negative pectin test indicates efficient viscosity reduction and good flocculation capacity, securing clean fermentation and reducing oxidation risks.

  • Sample: grape juice / must
  • Time required: ~10 minutes
  • Purpose: check pectin degradation before clarification or flotation

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How to do the pectin test

1. Prepare the acidified alcohol solution
Ethanol 96% acidified with 1% concentrated hydrochloric acid.

2. Prepare the must to be tested
If the must is charged with solid particles, a coarse filtration on paper is recommended.

3. Add the solution to the must
In a test tube, gently mix (to avoid breaking the pectin gel) 2 volumes of the prepared solution of acidified alcohol with 1 volume of must.

How to interpret the results

pectin test negative - no flocculation

Negative
Complete pectin hydrolysis

The solution remains clear after 10 minutes of reaction.

pectin test negative level 1

Positive
Low presence of pectin

When mixing, small bubbles are formed and slowly float upwards.

Pectin test positive level 2

Positive
Presence of pectin

When mixing, big bubbles are formed and can’t easily move up.

Pectin test positive - level 3

Positive
High presence of pectin

Heavy bubbles and a ring of gel
appear on the surface of the liquid after 10 minutes of reaction.


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