Fermentation management

In order for yeast to thrive and perform efficiently during fermentation, it is important to understand the nutritional requirements of yeast and how they can be met.

What is essential to evaluate for good yeast fermentation performance?

Evaluating the “yeast assimilable nitrogen” (YAN) level is important in winemaking because it can directly impact the yeast’s ability to grow and perform efficiently during fermentation.

The yeast assimilable nitrogen (YAN) level refers to the amount of nitrogen in the grape must that is usable by yeast. A low level of YAN may result in poor yeast growth and incomplete fermentation because the yeast will not have enough assimilable nitrogen to sustain their metabolism if the quantity is low. This can result in stuck or sluggish fermentations and lead to the production of off-flavours and aromas in the wine.

By measuring the YAN level in the grape must before fermentation, you can figure out if more nitrogen needs to be added to the must for the yeast to work at its best. There are different ways to measure YAN, like using a commercial assay or a simpler method.

You can manage the YAN level using Maxaferm. Because it contains yeast cell walls, DAP, and thiamine, this complex nutrient aids in mineral nitrogen supplementation, yeast viability, permeability of cell membranes, and cleaning the wine as it ferments.

Alcoholic fermentation follow-up with and without Maxaferm.
Maxaferm allows the end of fermentation at 21 days with no residual sugar and a steady fermentation. The control wine still contains 18 g/L of residual sugar after 26 days of fermentation. Inter Rhône, 2016.

How can we optimise the alcoholic fermentation?

Successful fermentation often results from the careful management of yeast nutrition throughout the fermentation. This may include ensuring that the initial yeast population is adequate, maintaining the optimum temperature and pH for the yeast, and supplying the yeast with the essential nutrients and oxygen.

When you are looking for greater aroma production, the elements apart from nitrogen should be considered, as should the nutrients containing yeast autolysates. We invite our section about yeast derivates production to understand the differences between the components, their properties,, and their use.  

We at Oenobrands have made a line of Natuferm products that focus on organic nutrition to improve the organoleptic quality of wines. These organic nutrients provide a balanced and comprehensive nutrient supply, ensuring that your fermentation process is both efficient and results in wines with superior organoleptic qualities. We invite you to read our section about wine aroma and wine modulation to get a better understanding.

And when fermentation slows down, what should be done?

When fermentation slows down, it is important to evaluate the health and nutritional status of the yeast. If the yeast population is low or stressed, adjusting temperature, aeration, and sugar levels may also help. 

A yeast revival product can be added to provide essential nutrients and boost yeast vitality.  This is the purpose of a yeast derivate like Extraferm D’tox, a pure yeast cell wall product. 

  • Fatty acids detoxification : When discussing fatty acids, we discuss the C6, C8, and C10 fatty acids, which inhibit yeast and lactic acid bacteria growth and block alcoholic and malolactic fermentations by making cell membranes impermeable to sugars. Extraferm D’tox at 20 g/hL reduces fatty acids in wine by 29%. This is after 24 hours of contact time with Extraferm D’tox making the wine more suitable for fermentation.
Levels of fatty acids in wine compared between the addition of Extraferm D’tox at 20 g/hL and no treatment.
  • Fast settling: A wine treated with Extraferm D’tox settles faster than a wine treated with other yeast cell walls. Yeast cell walls are added at 20 g/hL, but an Extraferm D’tox treatment settles in 45 minutes, whereas you would need double the time with other yeast cell wall products. This means that wine detoxified with Extraferm D’tox will be ready for the next production step earlier and with less risk of oxidation.
Turbidity in Wine: Comparing Extraferm D’tox and another cell wall

In extreme cases, a yeast strain change may be necessary to restart fermentation.

What do we suggest for restarting a stuck fermentation?

We have elaborated a protocol for the easy restart of a stuck fermention, involving the following products :

Here are the indications for restarting the 100 hL tank:

DAY 1:

DETOXIFICATION OF THE STUCK WINE

  1. Ensure the temperature of the tank is between 15-20 °C.
  2. Add SO2: 4-6 g/hL – If Total SO2 > 150 mg/l: add Delvozyme® (lysozyme) at 15-25 g/hL with 2 g/hL SO2
  3. Treat with Extraferm D’tox: 3-4 kg
  4. Allow it to settle for 24 hours before racking. 

DAY 2:

  • Fermivin CHAMPION BOOSTER Rehydration
  1. At 38 °C, dissolve 1.5 kg sugar in 50 L of water.
  2. Add 3 kg of Fermivin CHAMPION BOOSTER to this solution.
  3. Allow 30 minutes for rehydration.
  • Preparation of the restart inoculum 
  1. Add to the suspension of Fermivin CHAMPION BOOSTER rehydrated: 
    1. Water: 70 L (at room temperature)
    2. 12 kg sugar and 50 L stuck wine and mix
    3. 70 g of Maxaferm
  2. Let it cool down to 20–25 °C.
  3. Wait until the density reaches 1005 (maximum 24 hours).

DAYS 3 & 4:

ACCLIMATISATION of the RESTART INOCULUM

  1. Step 1: Add to the inoculum
    1. Stuck wine: 140 litres
    2. Water: 70 L (room temperature)
    3. Sugar: 25 kg
    4. Maxaferm: 180 g
  2. Wait for +/- 24 hours (or a density ≈ 995).
  3. Step 2: Add to the inoculum
    1. Stuck wine: 450 L
    2. Water: 50 L (room temp.)
    3. Sugar: 25 kg
    4. Maxaferm: 400 g
  1. Wait for +/- 24 hours (or a density ≈ 995)
  1. Add the acclimatised inoculum to the tank containing the previously detoxified and racked stuck wine.

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