Co-inoculation MLF

Co-inoculation malolactic fermentation is a technique that consists of co-inoculating a must with yeasts and malolactic ferments. It significantly accelerates the completion of malolactic fermentation. This time savings can be decisive for early wines and wines with a high pH (>3.6) and can limit the risk of deviations due to lactic bacteria or yeast contamination. Depending on the case, the lactic acid bacteria are added at the same time as the yeasts (co-inoculation in the strict sense) or after the yeasts at the start or during alcoholic fermentation (early bacterial seeding).

What are the benefits of doing MLF by co-inoculation?

Co-inoculation, where malolactic fermentation (MLF) occurs simultaneously with alcoholic fermentation (AF), offers several benefits in winemaking:

  • Shorter total MLF duration.
  • Efficient MLF in difficult wines. 
  • No MLF nutrition is required. 
  • Reduced SO2 usage. 
  • Less inhibitory impact from yeast (ethanol/medium-chain fatty acids). 
  • More ester production (fresh and fruity profile). 
  • Less diacetyl/buttery characters.
  • More complex, integrated wines. 
Bioprotection explanation doing MLF in co-inoculation with Anchor DUET bacteria. 
DUET Arom blend of bacteria in comparison with an O. oeni culture in co-inoculation MLF in Tempranillo (Spain). 
DUET Soft blend of bacteria in comparison with an O. oeni culture in co-inoculation MLF in Tempranillo (Spain). 

What are the important factors for co-inoculation?

To obtain a good success rate, it is important to follow several points:

  • Good management of yeast growth, development, and nutrition.
  • Good management of temperature
  • Avoid excessive sulphiting for white wines (seeding at 24 hours in the case of sulphiting < 5 g/hl, sowing at 48 hours in the case of sulphiting of 5 to 8 g/hL, sowing at 72 hours in the case of sulphiting > 8 g/hL) .
  • Regularly monitor malic acid during the last third of alcoholic fermentation
  • For white wines with high acidity, it is critical to account for the “natural” drop in pH during the first days of fermentation, as well as the yeast’s nitrogen compound metabolism. The pH then goes up at the same time that the yeast makes alcohol. Because of these factors, it is preferable to carry out bacterial seeding when approximately 50% of the alcoholic fermentation is complete.
  • Do not implement co-inoculation on spoiled grapes.

What difference can you find between the Anchor DUET bacteria?

It is possible to select a bacteria culture for MLF the same way you would select the yeast for AF, according to the sensory impact of the wine. Blended cultures of O. oeni and L. plantarum, like the Duet range from Anchor Oenology, provide both a secure fermentation as well as enhanced sensory characters. The L. plantarum strain can release bound aroma molecules from grape precursors and enhance floral and fruity characters during co-inoculation. Alternatively, bacteria blends can reduce the harshness and greenness of MLF, allowing for a softer wine style. Bacteria cultures from Anchor Oenology allow you to modify the wine flavour profile during MLF. 

The Duet Arom, Soft and Mature bacteria blends are focused on enhancing the quality, aroma and sensory perception of red, white and rosé wines during malolactic fermentation. 

Duet Mature in comparison with other commercial co-inoculation cultures, South African Cabernet sauvignon.

Discover the ANCHOR bacteria range

Anchor Oenology logo

Discover the MALOFERM bacteria range

MALOFERM-wine-bacteria-malolactic-bacterie-vin-couleur-350

What about you?

With a highly qualified team of experts in many fields, Oenobrands strives to offer winemakers’ novel and scientifically sound solutions, as well as to highlight the positive synergies between its products.

Search your solution or product

Tell us about your difficulties, and we will answer you

© 2024 - Oenobrands - Advanced winemaking solutions - Legal notice & Privacy policy