Tannic power

What are the tannins and how do they impact the texture?

In grapes, tannins are one of the most important families of compounds that eventually impart a wine’s mouthfeel.

Obviously, tannin composition is a key point, and winemaking processes strongly contribute to the final tannin’s composition, with reactions of addition, oxidation, polymerisation, and aggregation involving the native forms. However, the grape variety can influence the profile of the different phenolic families and, thus, the final composition of tannins in the wine and the sensory properties they induce. Note that the higher the degree of polymerisation and galloylation of tannins, the more astringent they are.

Impact of the tannin structure on the astringency perception.

The quantity of tannins, on the other hand, is more dependent on the maturation conditions.

These evolutionary mechanisms can lead to the preservation/formation of polyphenolic structures that present a high interaction with proteins, especially Proline Rich Proteins (PRPs), inducing the partial precipitation of protein-tannin structures. This reaction partly explains the decrease of wine astringency when protein fining reagents are used.

  • But the same mechanism also forms the basis of astringency in the mouth due to the loss of the lubrication effect of saliva when saliva PRPs are involved. This loss of lubrication is then perceived by the mechanoreceptors of the mouth mucosa, such as MUC1.
  • A second hypothesis is that the interaction with tannins occurs directly on the protein adherent to the epithelial mechanoreceptor MUC1, leading to a cleavage of one part of this protein that induces receptor activation (Canon et al., 2021). In this case, the reaction between tannins and saliva PRPs must be considered as a prevention mechanism, which is in line with the increase of PRP levels in the saliva of mammals subjected to a tannin-rich diet.

What about the grapes polysaccharides?

Similarly, grape polysaccharides that are extracted mainly during maceration and pressing indirectly contribute to the mouthfeel of wine. If grape polysaccharides are not abundant enough to be directly perceived in the mouth, they can prevent tannin aggregation or reduce their size.

These aggregates are known to be more astringent, and thus the presence of polysaccharides can reduce astringency.

Among the huge diversity of grape polysaccharides, rhamnogalacturonan II is the only compound that seems to have a direct and specific contribution to a decrease in astringency, avoiding the precipitation of tannin-protein structures in the mouth.

Polysaccharides and rhamnogalacturonan II clearly decrease wine astringency, as recently reported in statistical studies that combined polysaccharide composition and sensory.

On the contrary, the impact of oligosaccharides resulting from pectinase addition is not so clear, with controversial data highlighting the importance of the size and composition of mannose and galactose in those compounds.

What is the impact of yeast and bacteria on tannins?

Tannins can be directly absorbed by the yeast cell wall during alcoholic fermentation, thereby decreasing their level in wine and, correlatively, their higher presence in lees after alcoholic fermentation. This mechanism could partly explain the roundness of wine fermented with Fermivin XL in comparison to other strains.

Absorption of tannins by different yeast strains in red wine (data from INRAe).

What biotechnologies can be used post-fermentation to enhance the texture of the wine?

• Enzymes during ageing

Even if autolysis is a natural phenomenon occurring after alcoholic fermentation, it can be accelerated using β-glucanase enzymes. These enzymes can degrade the yeast cell wall since they are able to hydrolyse β-glucans that are constitutive of the cell wall. The effect of glucanase addition such as Rapidase Batonnage on mannoprotein release can be observed. The release of mannoproteins during lees ageing contributes to decreased wine astringency, increased unctuosity and mouthfeel, but also to tartaric, protein and colloidal stabilisation.

Mannoproteins (mg/L) release into wine ageing on lees. Untreated vs. Rapidase Batonnage MG 3g/hL, Chardonnay, France.
enzyme-Rapidase batonnage liquid-wine-mouthfeel
Mannoprotein release monitoring in a Glera wine (Italy, 2021).

• Yeast mannoprotein addition

Another way to take advantage of mannoproteins in wine is the addition of mannoproteins extracted from yeast and proposed as ready-to-use products, such as Claristar and Final touch which contribute to tartaric stabilisation and present a strong organoleptic impact. A full range of products are available with different applications depending on the yeast strain and extraction process used. A good example of the impact of such a mannoprotein is illustrated by the effect of Final touch GUSTO addition on tannin perception.

Tasting results of Tannins evaluation in percentage – compiled results of 6 red wines. Control vs. Final touch GUSTO.

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