Technical protocols for Rapidase Revelation Aroma

Rapidase protocols

Rapidase® Revelation Aroma is a high-purity glycosidase preparation engineered to act on the full range of glycosidically bound aroma precursors.
It is used in two distinct applications:

• Aroma release in any wine (terpenes, norisoprenoids)
• Smoke taint management, as the first step of smoke taint remediation by hydrolysing bound smoky compounds

Below are the operational protocols for each application.

Evaluate the aromatic impact of Rapidase Revelation Aroma through a controlled 6-week bottle trial.

• Use the same wine for all bottles.
• One bottle is kept as the untreated control (no enzyme added).
• The remaining six bottles will receive weekly enzyme additions.

For the test to remain representative, the enzyme solution is prepared in wine, not water, to avoid dilution.

Preparation:

• Dissolve 0.5 g of Rapidase Revelation Aroma in 500 mL of the wine being tested.
• Prepare a fresh solution every week.
• Each week, use 15 mL of this fresh solution per bottle.

For each of the six treatment bottles:

1. Add 15 mL of the freshly prepared enzyme solution.
2. Close the bottle immediately.
3. Store all bottles away from light, at cellar temperature (>13°C).
4. Repeat once per week for six consecutive weeks.

Each bottle corresponds to one contact time: Week 1 → Week 6.

Perform tasting one week after the last addition.

Evaluate for each bottle (control + weeks 1–6): colour, nose, palate, and observations.

Goal: identify when the aroma release reaches optimum expression relative to the control.

Once the preferred contact time is identified, apply 2 g/hL in the tank at cellar temperature, and after completion, inactivate with 5–10 g/hL bentonite.

Smoke-related volatile phenols exist in wine as glycosides. Using a complete glycosidase formulation will make available all the free forms of glucosides, which can then be removed.

Dose: 5 g/hL of Rapidase Revelation Aroma (Keep a volume of wine without treatment = control)

Contact time: Minimum 2 weeks in a tank > 16 °C Or 1 week at 25 °C in closed bottles with inert gas

Evaluation (vs control without enzyme addition):

• Tasting (easy but not accurate)
• Free smoke taints analysis (GC/MS analysis of guaiacol, 4-methylguaiacol, cresols and associated markers).

Product: Extraferm D’tox

Procedure:

• Add 20 g/hL of Extraferm D’tox, homogenise, and allow to settle.
• After 48 h, rack and add a second 20 g/hL, homogenise, and let settle.
• Rack off after 48 h

Treatment evaluation:

• Tasting
• Free smoke taints analysis (GC/MS analysis for final quantification)